Referenciák

A Bambanker sikerrel alkalmazható a legérzékenyebb sejtek fagyasztására is. Oldalunkon összegyűjtöttünk néhány sikeres alkalmazásról szóló közleményt

Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer

Attention: This is not the whole article but just an extract where the use of Bambanker was described

Takafusa Hikichi, Sayaka Wakayama, Eiji Mizutani, Yasuhiro Takashima, Satoshi Kishigami, Nguyen Van Thuan, Hiroshi Ohta, Hong Thuy Bui, Shin-Ichi Nishikawa and Teruhiko Wakayama 2007;25;46-53; originally published online Sep 28, 2006; Stem Cells

DOI: 10.1634/stemcells.2006-0439

Establishment of Parthenogenetic ES Cells

Parthenogenetic or cloned embryos at the morula or blastocyst stages were used to establish ES cell lines as described [1,34−36], with a slight modification in that 20% Knockout Serum Replacement (Invitrogen, Carlsbad, CA, http://www.invitrogen.com ) and 0.1mg/ml adrenocorticotropic hormone (American Peptide Company, Sunnyvale, CA, http://www.americanpeptide.com) were added to the multi ES cell medium instead of fetal calf serum [34]. Briefly, morulae/blastocysts were treated with acid Tyrodes solution to remove the zona pellucida and placed in 96-multiwell dishes coated with mouse embryonic fibroblasts (ICR origin) for at least 10 days. Proliferating outgrowths were dissociated using trypsin digestion and replated on fibroblasts until stable cell lines grew out. The culture medium was replaced with the cryopreservation medium Bambanker (NipponGenetics, Tokyo, http://www.n-genetics.com ), and the cell lines werestored in a 80°C freezer soon after being established, as described previously [37].

Runx2 deficiency and defective subnuclear targeting by pass senescence to promote immortalization and tumorigenic potential

Sayyed K. Zaidi, Sandhya Pande, Jitesh Pratap, Tripti Gaur, Simina Grigoriu, Syed A. Ali, Janet L. Stein, Jane B. Lian, Andre J. vanWijnen, and Gary S. Stein*

Department of Cell Biology and Cancer Center, University of Massachusetts Medical School and Cancer Center, 55 Lake Avenue North, Worcester, MA 01655; USA

Communicated by Sheldon Penman, Massachusetts Institute of Technology, Cambridge, MA, October 11, 2007 (received for review September 20, 2007)

[...]

Materials and Methods

Cell Culture: Primary calvarial osteoblasts were isolated from mouse embryos (17.5 days post coitum ) of WT or Runx2−null mice. Cells were frozen at passage 2 in BamBanker freezing medium for subsequent experiments. For experiments, cells were maintained in ?-MEM containing 10%FCS (HyClon), penicillin, streptomycin, and L-glutamine.